Effect of PYK_2 gene siRNA on proliferation of adult rat cardiac fibroblasts
- VernacularTitle:PYK_2小干扰RNA对成年大鼠心脏成纤维细胞增殖的影响
- Author:
Jia HAO
;
Jun YAN
;
Yingbin XIAO
- Publication Type:Journal Article
- Keywords:
protein-rich tyrosine kinase 2;
RNA interference;
cardiac fibroblasts;
proliferation
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To design and construct the vector of protein-rich tyrosine kinase 2 small RNA interference and investigate the effect of the recombinant plasmid on the proliferation of adult rat cardiac fibroblasts. Methods The pAVU6+27-PYK_2 siRNA expression vector was constructed by gene recombination, then transfected into the cultured adult rat cardiac fibroblasts by DOTAP method. PYK_2 mRNA and protein in cardiac fibroblasts were determined by RT-PCR and Western blotting respectively. Cell proliferation was tested by MTT and ~3H-TdR incorporation. Results The pAVU6+27-PYK_2 siRNA expression vector was successfully constructed. The recombinant plasmid can inhibit the proliferation of adult rat cardiac fibroblasts, as compared with control. Conclusion pAVU6+27-PYK_2 siRNA expression vector is efficient to suppress DNA synthesis of cardiac fibroblasts, which will be beneficial to further study of myocardial fibrosis.
