Relationship of caspase-3 activity and cardiac function in rat cardiomyocytes after myocardial reperfusion injury
- VernacularTitle:大鼠心肌再灌注不同时相caspase-3激活与心功能变化的关系
- Author:
Qiang XU
;
Liangyi SI
;
Hong ZHANG
- Publication Type:Journal Article
- Keywords:
myocardial ischemic reperfusion injury;
caspase-3;
apoptosis;
cardiac function
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between caspase-3 and left ventricular function at different phases after ischemia-reperfusion. Methods Totally 36 wistar rats were randomly divided into Sham operation group and ischemia-reperfusion(IR)group. The rat ischemic cardiac region of IR group was taken after 1, 3, 6, 12 and 24-hour reperfusion followed to 30-minute ischemia and detected for the activity fragment of caspase-3 P20, the activity of caspase-3, myocardial apoptosis index, left ventricular function (LVF) and creatine kinase-MB (CK-MB) respectively. Results With reperfusion time prolonging, the activity fragment of caspase-3 P20 increased, the activity of caspase-3, myocardial apoptosis index, left ventricular function and CK-MB all changed. The activity fragment of caspase-3 P20 and CK-MB peaked at 6 h, and the activity of caspase-3 and myocardial apoptosis index at 12 h, and then maintained platform; LVSP and ?dp/dt_max increased while LVEDP decreased gradually with the reperfusion time prolonging. All indexes mentioned above of IR group had statistical significance as compared with that of Sham operation group(P
