Studies on molecular mechanism of Anopheles anthropophagus resistance to deltamethrin Ⅲ. Semi-quantitative analysis of P450 genes mRNA between susceptible and deltamethrin-resistant strains of Anopheles anthropophagus
- VernacularTitle:嗜人按蚊溴氰菊酯抗性分子机理研究 Ⅲ 嗜人按蚊P450基因mRNA荧光半定量RT-PCR分析
- Author:
Jun CAO
;
Qi GAO
;
Junhong WANG
;
Huayun ZHOU
;
Julin LI
- Publication Type:Journal Article
- Keywords:
Anopheles anthropophagus;
Deltamethrin;
Insecticide resistance;
Real-time RT-PCR
- From:
Chinese Journal of Schistosomiasis Control
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between P450 (CYP6 and CYP4) genes and deltamethrin resistance in Anopheles anthropophagus. Methods The real-time RT-PCR technique was used for semi-quantitative analysis of mRNA copy numbers of CYP6 and CYP4 gene from susceptible and deltamethrin-resistant strains. Results It is found that the mRNA copy numbers of CYP6 gene in deltamethrin-resistant strain were about 1. 39 times higher than those in susceptible strain, and the mRNA copy numbers of CYP4 gene in deltamethrin-resistant strain were about 3. 63 times higher than those in susceptible strain of Anopheles anthropophagus. Conclusion The higher expressions of CYP6 and CYP4 mRNA are observed in deltamethrin resistance strain and indicate that both CYP6 and CYP4 genes may be involved in the deltamethrin resistance in Anopheles anthropophagus.
