Screening of promoter DNA-binding protein of cyclin B2 gene by phage display technique from human liver cDNA library
- VernacularTitle:应用噬菌体展示技术筛选细胞周期素B2启动子DNA的结合蛋白
- Author:
Jiang GUO
;
Jun CHENG
;
Longfeng ZHAO
- Publication Type:Journal Article
- Keywords:
phage display;
cyclin B;
promoter DNA binding protein screen
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen promoter binding protein of cyclin B2 by using human liver cDNA library, and investigate the expression and regulation mechanism of cyclin B2 gene. Methods By using cyclin B2 biotinylated promoter DNA as the selective molecule, the T7select human liver cDNA library was biopanned and positive clones were selected. After screening, positive plaques were performed to amplify for inserted DNA fragment, and the DNA fragment was cloned into pGEM-Teasy vector. Results Sequence analysis was performed in 20 positive plaques, and the full length sequences were obtained with bioinformatics method and searched for homologous DNA sequence from GenBank, altogether 6 coding sequences were obtained, all of which were known ones. Conclusion Cyclin B2 promoter binding proteins were screened. The results will be useful for further study the expression and regulation mechanism of cyclin B2.
