The influence of anti-sense hTERT on the expression of VEGF and Flt in SGC7901 cells
- VernacularTitle:反义hTERT对胃癌细胞SGC7901 VEGF及其受体表达的影响
- Author:
Yiling SI
;
Weidong HAN
;
Yali ZHAO
- Publication Type:Journal Article
- Keywords:
DNA, antisense;
human telomerase reverse transcriptase(hTERT);
stomach neoplasms;
vascular endothelial growth factors
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of anti-sense hTERT on the expression of VEGF and the receptor in SGC7901 cells, and study the influence in angiogenesis and progression in gastric carcinoma. Methods SGC7901 cell line was transfected by the recombinant virus containing sense and anti-sense hTERT cDNA. Then the expression of VEGF-C and Flt-4 was observed with RT-PCR, distribution of cell cycles was determined with flow cytometry. The expression of VEGF-C and Flt-4 protein was assessed with immunohistochemistry. Result The distribution of cell cycle of antisense hTERT transfected SGC7901 cells was changed, and the mRNA and protein expression of VEGF-C and Flt-4 was significantly down-regulated. Conclusion Anti-sense hTERT can act as an agent for inhibiting VEGF-C and Flt-4 mRNA and protein level, and it blocks tumor angiogenesis and lymphogenous metastasis.
