Rat spinal cord primary neuron injury-regeneration-related genes cloned by improved subtractive hybridization

VernacularTitle:应用锚定消减杂交技术克隆分析大鼠脊髓原代神经元损伤、修复相关基因
Author: Xin LI ; Haiping QUE ; Zhenlian MA
Publication Type:Journal Article
Keywords: neuron; injury and regeneration; gene; clone
From: Medical Journal of Chinese People's Liberation Army 2001;0(12):-
CountryChina
Language:Chinese
Abstract: Objective To clone injury-regeneration-related genes after primary neurons of spinal cord were injured in rat, and to elucidate the molecular mechanism of injury and regeneration in CNS. Methods An in vitro spinal cord primary neuron injury model was replicated in rat. The differentially expressed genes during injury and regeneration periods of spinal cord primary neurons were cloned by improved subtractive hybridization. Results Twenty-seven differentially expressed sequences were obtained, 23 were known genes and the others were unknown. Among these known sequences, cDNA for synuclein and clusterin, together with several other gene sequences, were closely related with nerve degenerative diseases. Conclusion Primary neuron injury-regeneration-related genes of the spinal cord can be successfully cloned by improved subtractive hybridization.