Influence of estrogen and progesteron on bone metabolism and renal expression of 1,25-dihydroxyvitamin D_3 receptors mRNA in ovariectomized rats
- VernacularTitle:雌、孕激素对去卵巢大鼠骨代谢及肾脏1,25-(OH)_2D_3受体mRNA表达的影响
- Author:
Yun ZHOU
- Publication Type:Journal Article
- Keywords:
bone and bones;
progesteron;
estrogen;
ovariectomy;
receptors,calcitriol
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influences of estrogen and progesteron on bone histomorphometry and renal expression of 1,25-dihydroxyvitamin D_3 receptor(VDR) mRNA in ovariectomized rats.Methods: Twenty-five adult female SD rats were equally randomized into 5 groups:sham-operation(Sham),ovariectomization(OVX),ovariectomization plus estrogen treatment(OVX+E),ovariectomization plus progesteron treatment(OVX+P) and ovariectomization plus both estrogen and progesteron(OVX+E+P) groups.All animals were killed to assess renal VDR mRNA expression by RT-PCR after 3.5 months feeding.Undecalcified proximal tibia was used to assess the bone histomorphometry with digitizing image analysis system.Results: OVX group,with weakened bone structure,had decreased cancellous bone and increased parameters of osteal formation and resorption compared with Sham,OVX+E and OVX+E+P groups(P
