Effect of photodynamic therapy with laser-activated BPD-MA on the expressions of mitochondria-related proteins Bcl-2 and Bax of human bladder cancer BIU-87 cells
- VernacularTitle:光动力作用对膀胱癌细胞线粒体相关调控蛋白Bcl-2/Bax表达的影响
- Author:
Chuanshan XU
- Publication Type:Journal Article
- Keywords:
laser irradiation;
photodynamic therapy;
bladder cancer cell;
apoptosis;
Bcl-2;
Bax
- From:Journal of Third Military Medical University
2003;0(22):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To study on the mechanisms that photodynamic therapy with laser-activated BPD-MA induces human bladder cancer BIU-87 cell apoptosis.Methods Human bladder cancer BIU-87 cells at log phase were randomized into 4 groups.The cells of BDP-MA-PDT groups were added with BPD-MA and then activated with 632.8 nm He-Ne laser;PDT group without BDP-MA addition was only irradiated;BDP-MA group without irradiation;normal control group without BDP-MA addition or irradiation.Photosensitization of BPD-MA was activated by laser with red light(632.8 nm) delivered at 10 mw/cm~(2) to give a total dose of 2.4 J/cm~(2).The expression of Bcl-2 and Bax proteins in human bladder cancer BIU-87 cells were detected by immunohistochemical staining.Results The Bcl-2 protein expression in BPD-MA-PDT group was lower than that of other groups(P0.05),therefore the ratio of Bax to Bcl-2 obviously increased(P
