Study on X-ray induced frequency of micronucleated erythrocytes in mice bone marrow by flow cytometer
- VernacularTitle:流式细胞仪检测X射线诱导小鼠骨髓红细胞微核率的初步研究
- Author:
Bingbing HU
;
Lujun YANG
;
Shengxue LIU
;
Jia CAO
- Publication Type:Journal Article
- Keywords:
X-ray;
polychromatic erythrocytes;
micronucleus;
flow cytometer;
biodosimeter for radiation
- From:Journal of Third Military Medical University
2003;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the frequency of micronucleated polychromatic erythrocytes(fMNPCE) in mice bone marrow after irradiated with X-ray by flow cytometer(FCM) to study whether fMNPCE detecting method could become a rapid biodosimeter for early radiation damage. Methods BALB/c mice were randomly assigned to receive the X-ray irradiation at dose of 0.5,3.0,6.0 Gy(n=4 for each irradiation dose),and the bone marrow was collected in 6,12,24 h after irradiation.Another 4 mice were intraperitoneally injected with cyclophosphamide twice as positive control,of which the bone marrow was collected in 42 h after intoxication began and 4 treated with spurious radiation as normal control.Results fMNPCE in mice bone marrow increased remarkably in 24 h after exposure to 0.5 Gy X-ray(P
