Inhibitory effect of panaxynol on the proliferation of rat aortic smooth muscle cell and its mechanisms
- VernacularTitle:人参炔醇对大鼠主动脉平滑肌细胞增殖的抑制作用及机制
- Author:
Liping JIANG
- Publication Type:Journal Article
- Keywords:
panaxynol;
rat aortic smooth muscle cells;
proliferation;
PDGF-BB;
intracelluar free Ca~(2+) concentration;
mtTF1
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the inhibitory effect of panaxynol(PNN) on the proliferation of rat aortic smooth muscle cell(RASMC) and its mechanisms.Methods Cell proliferation was determined using cell count and -TdR incorporation test.Fura-3/AM and confocal were used to measure intracelluar free Ca~(2+) concentration.Expression of mitochondrial transcription factor 1(mtTF1) mRNA was tested by using RT-PR.Results PNN inhibited the RASMC proliferation and DNA synthesis induced by serum and PDGF-BB in a dose-dependent manner.9 ?mol?L~(-1) of PNN inhibited the increase of intracelluar free Ca~(2+) concentration induced by PDGF-BB.PDGF-BB upregulated the expression of mtTF1 mRNA,which could be suppressed by 3,9 ?mol?L~(-1) of PNN significantly.Conclusions PNN can inhibit RASMC proliferation significantly,which might be related to the decrease of intracellular free Ca~(2+) concentration and mtTF1 mRNA expression.
