Effects of p38 MAPK kinase inhibitor SB203580 on the airway inflammation and Th2 cytokines in asthmatic mice
- VernacularTitle:p38蛋白激酶抑制剂SB203580对支气管哮喘小鼠气道炎症和Th2类细胞因子的影响
- Author:
Cuiping HUANG
;
Zhenxiang ZHANG
;
Heping YANG
- Publication Type:Journal Article
- Keywords:
p38 MAPK;
bronchial asthma;
airway inflammation;
cytokine
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of SB203580,a special inhibitor of p38 mitogen-activated protein kinase(p38 MAPK),on the airway inflammation and Th2 cytokines,interleukin 4(IL-4) and IL-5,in asthmatic mice.Methods Thirty mice were randomly divided into 3 groups: normal control,asthmatic group and SB203580 group.The expression of IL-4 mRNA and IL-5 mRNA in lung tissue and IL-4 and IL-5 protein in bronchial alveolar lavage fluid(BALF) were detected using in situ hybridization and ELISA,respectively.The number of inflammatory cells in BALF and histopathological changes were observed.Results The number of inflammatory cells and the concentrations of IL-4 and IL-5 in BALF,and the expression of IL-4 mRNA and IL-5 mRNA in lung tissue were higher in the asthmatic mice than in the normal control mice(P
