The protection effects of Panax Notoginsenosides on the gene expressionand protein excretion of TGF-?_1 and CTGF of human renal tubular epithelial cell induced by uremic serum
- VernacularTitle:三七总苷对尿毒血清诱导的人肾小管上皮细胞TGF-?_1、CTGF基因表达和蛋白分泌的影响
- Author:
Haiyan LIU
;
Xiaowen CHEN
;
Huafeng LIU
;
Dong LIANG
;
Deshen TANG
- Publication Type:Journal Article
- Keywords:
panax notoginsenosides(PNS);
uremic serum;
transforming growth factor-?_1;
connective tissue growth factor
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Panax Notoginsenosides(PNS) on the gene expression and protein excretion of TGF-?_1 and CTGF of human renal tubular epithelial cell induced by uremic serum in vitro.Methods Forty sera from CRF patients and twenty sera from healthy volunteers were collected,gently mixed,inactived and separated respectively in steriled condition.HK-2 Cells were cultured in RPMI-1640 medium with 10% newborn calves serum and subcultured routinely.They were differentiated by phase contrast microscope and scanning electron microscope detection and cytokeratin18(CK-18) immunohistochemistry method.The protein levels of TGF-?_1 were examined by enzyme-linked immunoadsordent assay(ELISA).The protein levels of CTGF were examined by Western Bloting assay.The gene expression of TGF-?_1 and CTGF was detected by Semi-quantitative reverse trainscriptase polymerase chain reaction(RT-PCR).Results TGF-?_1 and CTGF gene expression and protein level were increased in 10% uremic serum groups compared with that of normal control group(P
