Effect of Puerarin on calcium overload in cultured human umbilical vascular endothelial cells
- VernacularTitle:葛根素对培养人脐静脉内皮细胞钙超载的影响
- Author:
Guifeng XIAO
;
Gangmin NING
;
Yakun GE
;
Xiaoxiang ZHENG
- Publication Type:Journal Article
- Keywords:
puerarin;
calcium;
endothelial cell;
confocal laser scanning microscope
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To examine the effect of Puerarin on calcium overload in human umbilical vascular endothelial cells(HUVECs).Methods The HUVECs were incubated with Puerarin,then were exposed to H_2O_2,ATP and high K~+.The changes of intracellular free calcium([Ca~(2+)]i) in HUVECs were determined using fluorescent Ca~(2+)-indicator fluo-3/AM with confocal laser scanning microscope.Results Puerarin inhibited the elevation of [Ca~(2+)]i induced by H_2O_2,ATP and high K~+,and the inhibition ratio were 72.2%,56.5% and 78.2% respectively.The effect of Puerarin inhibiting the elevation of [Ca~(2+)]i induced by high K~+ was similar to that of verapamil.Conclusion The mechanism of puerarin reducing the overload of calcium in HUVECs may be related with blocking both voltage-dependent calcium channels and the release of calcium pool in endoplasm.
