Analysis of T-type correlation degree between bone mineral contents and histomorphometry of lumbar vertebrae body in ovariectomized rats
- VernacularTitle:去卵巢大鼠椎骨组织形态计量学与骨矿含量的T型关联度分析
- Author:
Bilian XU
;
Liao CUI
;
Tie WU
;
Yuyu LIU
;
Liyi ZOU
- Publication Type:Journal Article
- Keywords:
analysis of grey correlation;
T-type correlation degree;
osteoporosis;
bone histomorphometry;
bone mineral content
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the correlative changes of bone mineral contents and histomorphometry of lumbar vertebrae in ovariectomized rats by analysis of T-type correlation degree. METHODS: Forty 10.5-month-old virgin female Sprague-Dawley rats were randomly divided into five groups: (1) Basal: at 10.5 mon of age; (2) sham-1:sham-operated at 13 mon of age; (3) OVX-1: ovariectomized at 13 mon of age; (4) sham-2: sham-operated at 16 mon of age; (5) OVX-2:ovariectomized at 16 mon of age. After ovariectomy, all rats were treated orally with NS at 5 ml?kg -1?d -1. At the end-point of study, the undecalcified longitudinal fourth lumbar vertebra (LV4) sections were cut and stained with Goldner's Trichrome for bone histomorphometric analyses. The fifth lumbar vertebra (LV5) was dried with temperature and digested with acid for testing of bone mineral content. Then the effects of bone mineral contents on bone histomorphometry were assessed by analyzing the T-type correlation degree in the Grey system. RESULTS: All degrees of correlation between bone mineral contents and static histomorphometric parameters (trabecular bone volume) (BV/TV), trabecular thickness (Tb.Th) were positive, but for dynamic histomorphometric parameter (BFR/BV), the correlation degrees were negative. The effect of contents of calcium (Ca) and phosphorus (P) on histomorphometric parameters of lumbar vertebrae was much greater than that of the other bone mineral contents. CONCLUSIONS: Analysis of T-type correlation degree can evaluate the correlative changes of bone mineral contents and histomorphometry of lumbar vertebrae in ovariectomized objectively and fairly.
