Effect of chronic atrial fibrillation on Ca~(2+) /calmodulin dependent protein kinase expression in human atrial myocytes
- VernacularTitle:慢性心房颤动对人心房肌钙/钙调素依赖性蛋白激酶Ⅱ表达的影响
- Author:
Jinjin CHEN
;
Yingbin XIAO
;
Jian LIU
- Publication Type:Journal Article
- Keywords:
cardiac atrium;
atrial fibrillation;
calcium overload
- From:Journal of Third Military Medical University
2003;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of chronic atrial fibrillation on free calcium concentration and expression of Ca 2+ /calmodulin dependent protein kinase Ⅱ (CaMKⅡ) in human atrial myocytes. Methods The intracellular free calcium concentration in rapidly isolated atrial myocytes and the expression of CaMKⅡ in atrial tissue of rheumatic heart disease patients with atrial fibrillation (AF) or with normal sinus rhythm were measured by laser scanning confocal microscopy and Western blotting respectively. Results The intracellular Ca 2+ concentration of patients with atrial fibrillation was significantly higher than that of patients with normal sinus rhythm [(276.38?38.12) vs (122.28?45.63) nmol/L, (P
