Effect of caffeic acid phenethyl ester on the growth of human colorectal carcinoma cell line HCT116 transplanted subcutaneously in nude mice
- VernacularTitle:咖啡酸苯乙酯对人大肠癌HCT116细胞裸鼠皮下移植瘤生长的影响
- Author:
Debing XIANG
;
Dong WANG
;
Jianghong MOU
- Publication Type:Journal Article
- Keywords:
caffeic acid phenethyl ester;
colorectal neoplasms;
apoptosis;
neoplasm transplantation
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of caffeic acid phenethyl ester (CAPE) on the growth of human colorectal carcinoma cell line HCT116 transplanted subcutaneously in nude mice. Methods Nude mouse model of human colorectal carcinoma by subcutaneous transplantation of HCT116 cell line was reproduced. A total of 20 mice were divided into 2 groups: control group and CAPE group (oral administration of CAPE at 5mg/mice/d). The growth of the subcutaneously transplanted tumor and changes in mouse body weight in each group after treatment were observed on 7, 14, 21 and 28d. Histopathological examination of xenograft, heart, liver, lung, kidney and intestine of nude mice was also conducted. Apoptosis index was detected by terminal dUTP nick end labeling (TUNEL) technique. Results CAPE had significantly inhibitory effect on growth of the transplanted xenograft in vivo. Tumor volume and tumor weight were decreased (P
