Research on multiple-drug resistance expression of CNE1 and CNE2 cell lines induced by radiation
- VernacularTitle:辐射诱导人鼻咽癌细胞CNE1、CNE2多药耐药的研究
- Author:
Hai SUN
;
Faquan LIU
;
Juyi WEN
- Publication Type:Journal Article
- Keywords:
nasopharyngeal neoplasms;
radiation;
multidrug resistance;
cyclosporine A;
interferon-alpha
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study multiple-drug resistance expression of CNE1 and CNE2 cell lines induced by radiation and its reversal by cyclosporine A and interferon. Methods Drug-resistant CNE1 and CNE2 cell lines were induced by radiation, and expression of P-gp, MRP, TOPⅡ and GST-? on protein and mRNA level were analyzed by flow cytometry and reverse transcription-polymerase chain reaction methods before and after radiation. At the same time, effect of cyclosporine A and interferon on multiple-drug resistance expression induced by radiation was studied. Results The results showed that expression of P-gp, MRP and GST-? proteins on CNE1 and CNE2 cell lines were up-regulated by radiation, and cyclosporine A and interferon could reverse the up-regulation of P-gp and MRP proteins induced by radiation. Conclusion Radiation can induce multiple-drug resistance in CNE1 and CNE2 cell lines, and it can be reversed by cyclosporine A and interferon.
