Influence of tumor suppressor gene p33~(ING1b) on growth of pancreatic carcinoma cell line SW1990
- VernacularTitle:正反p33~(ING1b)对人类胰腺癌细胞SW1990生长的影响
- Author:
Guanzhen YU
;
Ying CHEN
;
Zhi ZHU
;
Minghua ZHU
- Publication Type:Journal Article
- Keywords:
pancreatic neoplasms;
p33~ING1b;
wtp53;
apoptosis
- From:
Academic Journal of Second Military Medical University
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of p33~ING1b and wt-p53 on the biological activities of pancreatic cancer cells. Methods: Sense and anti-sense cDNAs of p33~ING1b were cloned into pcDNA3 eukaryotic expression vector separately, and the recombinant plasmids were solely or co-transfected with wt-p53 into pancreatic cell line SW1990. Flow cytometry was used to analyze the changes of cell cycle, Western blotting was used to evaluate the expression of p33~ING1b and p53 protein, and MG-P-MY staining was applied to observe cell apoptosis. Results: SW1990 cells solely transfected with sense p33~ING1b plasmid or co-transfected with wt-p53 and sense p33~ING1b plasmid expressed more p33~ING1b and had more apoptosis than the antisense p33~ING1b plasmid and mock transfected cells did (P
