Corticotrophin-releasing hormone promotes estrogen synthesis in human placentas
- VernacularTitle:促肾上腺皮质激素释放激素对胎盘雌激素合成的促进作用
- Author:
Ruifang YANG
;
Xiaolu TANG
;
Xin NI
- Publication Type:Journal Article
- Keywords:
corticotrophin-releasing hormone;
estrogen;
placenta;
estradiol;
P-450arom
- From:
Academic Journal of Second Military Medical University
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of corticotrophin-releasing hormone(CRH) on estrogen production in human trophoblasts and its mechanisms. Methods: Cytotrophoblasts were isolated from term human placentas and cultured for 72 h. The cultured cells were then treated with various concentrations of CRH and CRH receptor antagonist,?-Helical CRH9-41 for 24 h; estradiol was measured by radioimmunoassay in the culture media. Expression of aromatase(P450arom),the key enzyme for estrogen synthesis,was analyzed by Northern blot. Results: CRH significantly stimulated estradiol production and the expression of P450arom mRNA in placental cells. It was found that ?-Helical CRH9-41 blocked the effect of CRH and inhibited estradiol production and P450arom mRNA expression(P
