Increased expression of transforming growth factor-beta 1 in fetal liver stromal cells induced by hypoxia
- VernacularTitle:低氧诱导胎肝基质细胞表达转化生长因子?1表达升高
- Author:
Gexiu LIU
;
Yuan ZHANG
;
Dongmei HE
;
Guangxiao TAN
- Publication Type:Journal Article
- Keywords:
hypoxia;
transforming growth factor-?1;
embryo;
liver;
stromal cell
- From:
Academic Journal of Second Military Medical University
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the expression of transforming growth factor-?1 (TGF-?1) in fetal liver stromal cells during hypoxia,in an attempt to provide evidences for cell therapy of brain ischemia. Methods: Cells from 14.5-day-old mouse fetuses were cultured and passaged. Subconfluent cells of the fifth passage were cultured in hypoxia condition (95% N2 + 5% CO2 ). TGF-?1 mRNA and TGF-?1 were detected by semi-quantitative RT-PCR and Western blot,respectively. Results: TGF-?1 mRNA and TGF-?1 levels,which are very low in normally cultured cells, increased significantly in hypoxia-cultured cells. Expression of TGF-?1 mRNA increased from (5.47?2.76)% to (10.38?3.65)% at 4 h of hypoxia(P
