Effect of intraperitoneal injection of IFN? on GATA-3 expression and airway inflammation in lungs of mice with asthma
- VernacularTitle:腹腔注射干扰素?对哮喘小鼠肺组织GATA-3及气道炎症的影响
- Author:
Yan HUANG
;
Jiquan CHEN
;
Qingyu XIU
- Publication Type:Journal Article
- Keywords:
interferon gamma;
asthma;
GATA-3;
injections,intraperitoneal
- From:
Academic Journal of Second Military Medical University
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the preventive and therapeutic effects of intraperitoneal injectoin of IFN? on bronchial asthma in mice and the relevant mechanism. Methods: Thirty-six BALB/c mice were randomly equalized into 3 groups:group A (normal control group),group B (asthmatic model group) and group C (IFN? treated group). The asthmatic model was established in group B and C by immunization with ovalbumin (OVA) absorbed to aluminum hydroxide. Mice of group B and C received 0.25 ml PBS and 5 ?g IFN? intraperitoneally on days 23 to 30 once daily prior to ovalbumin challenge,respectively. Bronchoalveolar lavage fluid (BALF) was collected on day 31 for determining the cellular composition and the concentrations of IL-4 and IL-5. Meanwhile,IgE in serum was determined. The pathological changes and the expression of GATA-3 were investigated in the lungs of mice. Results: (1) BALF eosinophils was significantly decreased in group C compared with those in group B ( vs ,P
