Ultrasonic condition for extraction of flavone from Radix Astragali by orthogonal design
- VernacularTitle:正交设计法确定黄芪中黄酮类成分的超声提取条件
- Author:
Songgang JI
;
Xiang LI
;
Ziyang LOU
;
Bin WANG
;
Yifeng CHAI
- Publication Type:Journal Article
- Keywords:
orthogonal design;
Radix Astragali;
flavonoids;
isolation & purification
- From:
Academic Journal of Second Military Medical University
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To ascertain the optimized ultrasonic condition for extraction of flavone from Radix Astragali by orthogonal design. Methods: The contents of calycosin-7-O-?-D -glucoside and formononetin were taken as the indices and were determined. The ultrasonic time (10 min, 20 min, and 30 min), concentrations of methanol (50%, 75% and 100%) and times of extraction (1, 2, and 3) were analyzed by orthogonal design; the best ultrasonic condition was ascertained and compared with those of soak extraction and Soxhlet extraction. Results: Ultrasonic with 100% methanol twice (20 minutes each time) was the optimized condition for extraction of flavone from Radix Astragali. The efficiency of ultrasonic extraction was better than those of soak extraction and Soxhlet extraction. Conclusion: Compared with other methods, the ultrasonic extraction of flavone from Radix Astragali is efficient, quick and simple.
