Transdifferentiation of hair follicle bulge cells into corneal epithelial cells induced by corneal limbal stroma in vitro
- VernacularTitle:毛囊bulge细胞在角膜缘基质诱导下向角膜上皮细胞分化的初步研究
- Author:
Jin YU
;
Ke YANG
;
Tian YANG
;
Gang FU
- Publication Type:Journal Article
- Keywords:
hair;
bulge cell;
cornea;
epithelium;
transdifferentiation
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of the transdifferentiation of hair follicle bulge cells into corneal epithelial cells in vitro. Methods The hair follicle bulge cells from 7- to 8-day SD rats were isolated and cultured in vitro, then co-cultured with rabbit corneal limbal stroma in transwell-cultured system. The differentiation and development of hair follicle bulge cells were observed, and immunocytochemical staining were used to detect expression of K19 and K12 in hair follicle bulge cells. Results The cultured bulge cells possesed high proliferation and low differentiation, co-expressed K19 and ?_ 1 integrin, but part of them expressed K12 after 2-week co-culture with rabbit corneal limbal stroma in transwell-cultured system. Conclusion Rat hair follicle bulge cells could transdifferentiate into corneal epithelial cells induced by corneal limbal stroma in vitro.
