Detection and significance of apoptosis related factors in the primary human embryonic pancreatic islet cells
- VernacularTitle:原代人胎胰岛细胞凋亡相关因子的检测及其意义
- Author:
Bing CHEN
;
Haihui WANG
;
Haitao GUO
;
Yumin WANG
- Publication Type:Journal Article
- Keywords:
diabetes;
pancreatic islet cells;
apoptosis
- From:Journal of Third Military Medical University
2003;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between TGF-?, P21ras, c-fos and the apoptosis of human embryonic pancreatic islet cells. Methods Semi-quantitative RT-PCR and immunohistochemistry (SABC method) were used to detect TGF-?, P21ras, c-fos expression at different growth periods of the primary human embryonic pancreatic islet cells and human fibroblast cells. Results P21ras expression was weak in every period of the pancreatic islet cells and significantly weaker than the fibroblast cells at exponential growth period and was stronger than in incubation period and retention period (P
