Protective effects of baicalin on Neuro2A cells under oxygen and glucose deprivation
- VernacularTitle:黄芩苷对Neuro2A细胞氧糖剥夺的保护作用
- Author:
Xia WANG
;
Pt CHEUNG
;
Le ZHONG
;
Xiaohe YU
;
Yujia YANG
;
Guangjian CHEN
- Publication Type:Journal Article
- Keywords:
Neuro2A cells;
ischemia;
anoxia;
baicalin
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim Neural injury in the central nervous system following is chemic insult is believed to result from oxygen and glucose deprivation.In this study,baicalin was investigated for its neuroprotective effects against oxygen and glucose deprivation(OGD) in Neuro2A cells.Methods Mitochondrial activity was assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) reduction activity assay.Apoptosis was monitored with flow cytometry.It was found that baicalin increased MTT reduction activity and decreased percentage of apoptosis of the cultured Neuro2A cells.Results Baicalin showed significant protective effects on the OGD-induced apoptosis in cultured Neuro2A cells.Conclusion These results suggest that baicalin is an effective compound in preventing neurotoxicity induced by OGD and therefore deserves further scrutiny.
