A neonatal mouse model of AIDS induced by L6565 murine leukemia virus
- VernacularTitle:应用鼠白血病病毒L6565建立小鼠艾滋病模型
- Author:
Min JIA
;
Tiehong YANG
;
Xiujuan YAO
;
Jia MENG
;
Qibing MEI
- Publication Type:Journal Article
- Keywords:
AIDS,retrovirus;
immuneomodulation
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish a mouse model of AIDS and observe the effects of Combivir,an anti-AIDS drug.Methods The model was set up by infecting infant mice with L6565 murine leukemia virus.Quantitative RT-PCR method was used to measure viral load in plasma.The percentage of CD4~+ and CD8~+ lymphacytes was estimated by flowcytometric method.The number of WBC,RBC and marrow karyote was counted.indexes of immune organs were weighed and calculated.Results Compared with control,the viral load in plasma in the model was at high level.The percentage of CD4~+ lymphocyte,the ratio of CD4~+/CD8~+,the number of RBC and marrow karyote,and thymus index were significantly decreased in the model,while the number of WBC and spleen index was remarkably increased.Conclusion AIDS patient symptom is shown in mouse infected with L6565 murine leukemia virus.This suggests that this model is likely to be used as a mice model to evaluate the effect of anti-AIDS drugs.
