Chronic subcutaneous injection of rotenone produces rodent Parkinsonian models
- VernacularTitle:长期低剂量皮下注射鱼藤酮制作大鼠帕金森病模型
- Author:
Xilin ZHAO
;
Zhenlun GU
;
Zhenghong QIN
- Publication Type:Journal Article
- Keywords:
rotenone;
parkinsons disease;
substantia nigra;
tyrosine hydroxylase;
?-synuclein
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish a rat model of Parkinsons disease by chronic subcutaneous injection of low-dose rotenone.Methods Lewis rats were randomly divided into two groups: vehicle-treated group and rotenone-treated group.Rotenone(1.0 mg?kg~(-1)?d~(-1)) was subcutaneously injected at 08:00 and 20:00 for 30 days with drug holidays every 7 days.Following 30 days of rotenone administration,neuronal loss in the substantia nigra(SN) was determined with tyrosine hydroxylase(TH) immunohistochemistry and Nissl staining.Aggregation of ?-synuclein in SN neurons was observed with a laser cofocal microscopy.Result Three rotenone-treated rats showed resting tremor.The number of TH-positive neurons in SN significantly reduced(P
