Comparison of gene expression and DNA immunization efficacy encoding prME and E proteins derived from Japanese encephalitis virus
- VernacularTitle:乙型脑炎病毒prME、E蛋白基因表达与DNA免疫效率对比研究
- Author:
Guohe FENG
;
Yumei WANG
;
Pei LIU
- Publication Type:Journal Article
- Keywords:
Encephalitis virus,Japanese;
Viral proteins;
Gene expression;
Immunoblotting;
Neutralization tests;
Vaccines,DNA
- From:
Chinese Journal of Infectious Diseases
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
3 ?g/well.All BALB/c mice immunized with pJME by im.and gene gun injection,and JE inactivated vaccines by ip.injection survived for 21 days.BALB/c mice immunized with pJE by im.injection and gene gun injection partially survived for 21 days.Titres of neutralization antibody produced with pJME were higher than pJE.Protective immunity and titre of neutralization antibody produced by im injection was the same as gene gun injection(im/gene gun injection: 1∶320/1∶320) at day 21.The antibody from BALB/c mice sera after twice pJME immunization only reacts with JEV-E protein. Conclusions Expression efficacy of proteins encoded by pJME and pJE in transfected cells is different.Expression level of related proteins was dependant on recombinant amount for transfection in a certain degree.Immunity effect induced with pJME was higher than pJE.The efficacy of DNA immunization produced by gene gun injection was higher than im.injection.Titres of neutralization antibody induced by DNA immunization were correlated to efficacy of protective immunity.Neutralization antibody from BALB/c mice sera produced by pJME immunization contained anti-E antibody against JEV-E protein.
