Protection effect of ?_1 receptor mRNA antisense inhibition on heart of renal hypertensive rats
- VernacularTitle:?_1受体反义基因治疗对肾性高血压大鼠心脏的保护作用
- Author:
Yuanxiong WU
;
Jinming WANG
;
Meichun ZHANG
;
Yuanhong LIANG
- Publication Type:Journal Article
- Keywords:
renal hypertension;
antisense oligonucleotides;
?_1-adrenergic receptor;
gene therapy;
left ventricular hypertrophy;
heart protection;
apotosis;
Bcl-2/Bax
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
ATM: To explore the effect and mechanism on blood pressure and heart protection of renal hypertension in rats treated with ?_1 receptor antisense oligodeoxynucleotides (?_1-AS-ODN) by delivery with cationic liposomes DOTAP/DOPE. METHODS: 24 SD rats were divided randomly into 4 groups of 6 each group: two groups of 2K1C rats were treated via tail vein injection with ?_1-AS-ODN or inverted oligonucleotides against rat ?_1-AR mRNA (?_1-IN-ODN) of 0.5 mg?kg~(-1) once. 6 untreated 2K1C rats and shamed rats served as positive and normal controls. Blood pressure (BP), cardiac haemodynamics, and left ventricular index (LVW/BW) were measured. The histological changes of myocardium were observed by optical microscope and the expressions of Bcl-2 and Bax in myocardium were examined by immunohistochemical method. RESULTS: The single dose management of ?_1-AS-ODN decreased blood pressure to 120 mmHg for 27 days and improved the LV function obviously (P
