THE DYNAMIC CHANGES OF PLASMA AND ERYTHROCYTE RIBOFLAVIN AND ITS DERIVATIVES CONCENTRATIONS IN RIBOFLAVIN DEFICIENT RATS
- VernacularTitle:核黄素缺乏大鼠血浆与红细胞核黄素及衍生物浓度的变化
- Author:
Qishou XU
;
Jingyu WEI
;
Jijun YANG
;
Jianyun YANG
;
Yonghui LI
;
Jing XU
- Publication Type:Journal Article
- Keywords:
riboflavin;
BGRAC;
HPLC
- From:
Acta Nutrimenta Sinica
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the dynamic changes of plasma and erythrocyte riboflavin and its derivatives concentrations after riboflavin deficiency and so as to provide theoretic basis for their application in riboflavin status assessment. Methods: The riboflavin deficiency was induced in rats by feeding a riboflavin deficient diet. The plasma and erythrocyte riboflavin and its derivatives (FMN and FAD) were analysed with HPLC method. The blood glutathione reductase activity coefficient (BGRAC), erythrocyte GSH concentration and urinary riboflavin excretion were also measured. Results: The plasma riboflavin concentration as well as the urinary riboflavin excretion were decreased rapidly early after riboflavin deficiency and followed by plasma riboflavin derivatives. The erythrocyte riboflavin and its derivatives and GSH concentrations were decreased significantly later. On the other hand, the BGRAC was increased significantly in the early period of riboflavin deficiency and the change, however, was limited in a narrow range. Conclusion: The plasma and erythrocyte riboflavin concentration can be applied as a marker in riboflavin status assessment but human studies are necessary for further research.
