Restriction Fragment Length Polymorphism of Tumor Necrosis Factorbeta Gene in Schizophrenia.
- Author:
Yoo Jin PARK
1
;
Taeyoun JUN
;
Chi Un PAE
;
Chung Tai LEE
;
Tae Yul LEW
Author Information
1. Department of Psychiatry, College of Medicine, The Catholic University of Korea, Seoul.
- Publication Type:Original Article
- Keywords:
Schizophrenia;
Tumor necrosis factor beta(TNFB) gene;
Polymorphism
- MeSH:
Alleles;
Diagnostic and Statistical Manual of Mental Disorders;
DNA;
Humans;
Molecular Biology;
Necrosis*;
Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length*;
Schizophrenia*;
Stem Cells
- From:Journal of Korean Neuropsychiatric Association
2001;40(2):341-347
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Schizophrenia is known to have high genetic influences. Recently, the main focus of etiologic study in schizophrenia has been concentrated on molecular genetic approach including polymorphism analysis. This study was designed to investigate the relationship between schizophrenia and immunologic influences by analyzing polymorphism of TNFB that is involved in interaction between immunologic system and CNS. METHOD: 146 schizophrenic patients diagnosed by DSM-IV criteria were included and data of 206 normal population from the Catholic Hemopoietic Stem Cell Information Bank(Seoul, Korea) were used as a control group in this study. DNA was extracted from whole blood, thereafter amplified by polymerase chain reaction, and digested by NcoI. We obtained and assessd RFLP of two alleles, TNFB1 which has a NcoI restriction site generating 555bp and 185bp fragments, and TNFB2 which lacks the NcoI restriction site. All data were analyzed by K 2 test. RESULTS: There were no significant differences in frequency of TNFB1/1, TNFB1/2, and TNFB2/2 between the schizophrenic patient and the control group. Alleric frequencies of TNFB1 and TNFB2 were significantly different between schizophrenic patient and control group. CONCLULSION: We found the possible association between alleles of TNFB and schizophrenia in this study. To clarify the influences of TNFB on schizophrenia, further systematic studies should be conducted.
