EFFECT OF SOYBEAN ISOFLAVONE ON PROLIFERATION AND EXPRESSION OF HIF-1? IN GASTRIC CANCER CELL SGC-7901 DURING HYPOXIA
- VernacularTitle:缺氧条件下大豆异黄酮对胃癌细胞增殖和缺氧诱导因子-1?表达的影响
- Author:
Junwen ZHANG
;
Pilong WANG
- Publication Type:Journal Article
- Keywords:
hypoxia;
soy bean isoflavone;
gastric cancer cell SGC-7901;
hypoxia-inducible factor-1?
- From:
Acta Nutrimenta Sinica
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objetive: To study the effect of soybean isoflavone(SI) on the growth of gastric cancer cell SGC-7901 during hypoxia and its mechanisms. Method: The environment of hypoxia was established by GasPaK method. The effect of SI was determined by MTT and FCM which was used for studying apoptosis and cell cycle of SGC-7901. Electron microscopy was used to study the effects of SI on apoptosis and necrosis of SGC-7901. Cell-immunochemistry was used to observe the change of hypoxia-inducible factor HIF-1? and FAS expression. Results: SI could inhibit significantly the growth of SGC-7901, and arrest the cell in G2/M phases during normal and hypoxia environment. The inhibitory effect was elevated significantly during hypoxia. Electron microscopy showed the cell apoptosis and necrosis. FAS expression was elevated by SI during normal and hypoxia environment, and HIF-1? expression was downregulated during hypoxia. Conclusion: SI can inhibit SGC-7901 cell growth via arrest of cell cycle, elevated expression of FAS, apoptosis and necrosis induction and other pathways. The inhibition of the increased HIF-1? expression is probably the mechanism of the inhibitory effects of SI during hypoxia.
