EFFECT OF BLACK CURRANT ON PROLIFERATION AND APOPTOSIS OF HUMAN ESOPHAGEAL CANCER CELL LINE IN VITRO
- VernacularTitle:新疆天然植物黑加仑对食管癌细胞增殖、凋亡的影响
- Author:
Xiaomei LU
;
Yalou ZHANG
;
Yan ZHANG
;
Hui XIAO
;
Yueming ZHANG
;
Hao WEN
- Publication Type:Journal Article
- Keywords:
Black currant;
human esophageal squamous cancer cell Eca109 line;
apoptosis
- From:
Acta Nutrimenta Sinica
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of black currant extract on human esophageal squamous cancer cell Eca109 line to reveal its probable mechanism. Methods: The survival cells and protein synthesis of tumor cell lines treated with 10-1, 10 -2,10-3 g/ml of the water extracts of black currant for 24 h were determined by MTT and Bradford assays, and the cell-DNA ploidy distribution and apoptotic rate were measured by flow cytometry (FCM) and morphological observation. Results: There was significant inhibition of the cell survival and protein synthesis of cancer cells at dose of 10-1 g/ml of the extracts. In the normal rat liver cells, the cell survival was not affected. An apoptosis peak appeared before diploid peak in FCM and apoptotic rate was 49.6%, and morphological change of apoptosis was observed. Conclusion: Black currant could significantly inhibit growth of human esophageal squamous cancer cell Eca109 line through inducing apoptosis.
