Role of the IGF/HGF/Gln on the culture of the intestinal organoid in vitro
- VernacularTitle:不同细胞因子在小肠类器官片断体外培养中的作用研究
- Author:
Dongnan HE
;
Qin HAN
;
Ning LI
;
Lianming LIAO
;
Weiming ZHU
;
Baojun YU
;
Gefei WANG
;
Chunhua ZHAO
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
Insulin-like growth factor;
Hepatocyte growth factor;
Glutamine;
Organoid
- From:Parenteral & Enteral Nutrition
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the nutritious effects of IGF, HGF and Gln on intestinal mucosal precursor cells in vitro.Methods: Different combinations of IGF,HGF and Gln were used to observe their effects on the expansion and development of the cultured intestinal mucosal precursor cells.Results: The combination of IGF,HGF and Gln could markedly promote the expansion of cultured intestinal mucosal precursor cells.Although IGF or HGF alone could promote the expansion of intestinal mucosal precursor cells, they had little effects on the development.On the contrary,Gln alone can promote the development of intestinal mucosal precursor cells,but it had little effects on their expansion.Conclusion: IGF,HGF or Gln alone has little effects on the expansion and development of the intestinal mucosal precursor cells.When they are used together,they can efficiently provide the essential stimuli for the expansion of the cultured intestinal mucosal precursor cells in vitro and enable the recombination of the different cells and formation of intestinal mucosa-like structure in vitro.
