Expression of novel apoptosis-related protein PDCD5 in granulosa cells of polysystic ovary syndrome
- VernacularTitle:多囊卵巢综合征患者黄素化颗粒细胞中PDCD5蛋白的表达
- Author:
Chunling SUN
;
Jie QIAO
;
Zhenxing HU
;
Ting ZHANG
;
Yingyu CHEN
- Publication Type:Journal Article
- Keywords:
Polycystic ovary syndrome;
Apoptosis;
Granulosa cells
- From:
Journal of Peking University(Health Sciences)
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect the novel apoptosis-related protein PDCD5 expression in granulosa cells of polysystic ovary syndrome(PCOS) and normal ovary, and explore the pathogenesis of PCOS. Methods:The granulosa cells were collected from 30 cases of PCOS and normal ovary in IVF-ET. Expression of PDCD5 was detected by flow cytometry; immunofluorescence and immunohistochemistry. Cell apoptosis was detected by Propidium Iodide (PI) staining. Results: The number of hypodiploidy cells associated with apoptosis in granulosa cells of PCOS was greater than that of the normal control. PDCD5 protein expression in PCOS granulosa cells was significantly higher than that in normal ovary(P
