Preparation,release and immunogenicity evaluation of HBsAg-PLGA microspheres
- VernacularTitle:乙型肝炎病毒表面抗原-乳酸/乙醇酸共聚物缓释微球的制备、释放和免疫学研究
- Author:
Li FENG
;
Xingjun ZHOU
;
Xianron QI
- Publication Type:Journal Article
- Keywords:
Hepatits B surface antigens;
Microspheres;
Sustained-release preparations
- From:
Journal of Peking University(Health Sciences)
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate microencapsulation technique, release of HBsAg-PLGA microspheres and degradation of the polymer in vitro, and the level of immune response after the single-dose HBsAg-PLGA microspheres subcutaneously injected (sc) to BALB/c mice. Methods: HBsAg-PLGA microspheres were prepared by double emulsion microencapsulation technique with orthogonal experiments. The pharmaceutical characteristics of size and surface morphology, antigen loading efficiency, release of HBsAg-PLGA microspheres and degradation of the polymer in vitro, and the level of immune response after single sc of PLGA microspheres in BALB/c mice were investigated. Results: The concentration of PVA was the significant factor affecting the particle size (P
