Construction and expression of recombinant plasmid pEGFPC1uPAR in Pam 212 cells
- VernacularTitle:pEGFPC1-uPAR重组质粒的构建及对细胞增殖和侵袭性的影响
- Author:
Qiangguo GAO
;
Gang FU
;
Yijun ZENG
;
Tian YANG
- Publication Type:Journal Article
- Keywords:
human uPAR;
pEGFPC1;
Pam 212 cells
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the eukaryotic expression plasmid of pEGFPC1uPAR gene and explore the effect on the proliferation and invasion ability of Pam 212 cells. Methods The human uPAR cDNA was cloned by PCR, and inserted into the eukaryotic expression plasmid pEGFPC1. After identification of sequencing, the reconstructive plasmid was transformed transiently into Pam 212 cells, then the cell growth and the invasion ability were evaluated. Results The reconstructive plasmid of pEGFPC1uPAR was validated by sequencing. The reconstructive plasmid can promote the growth of Pam 212 cells and enhance the invasion ability. Conclusion The pEGFPC1uPAR plasmid was constructed successfully and uPAR was confirmed to promote the growth and the invasion ability of Pam 212 cells, which lay the foundation for further studies of uPAR in vivo.
