Enhancement of AdhuVEGF_(121) gene transfection to ALP activity and collagen Ⅰ expression in femoral head of rabbits
- VernacularTitle:Ad-huVEGF_(121)基因转染对兔股骨头Ⅰ型胶原表达及ALP活性的影响
- Author:
Yong JIA
;
Tongwei CHU
;
Yue ZHOU
- Publication Type:Journal Article
- Keywords:
vascular endothelial growth factor;
alkali phosphatase;
collagen Ⅰ
- From:Journal of Third Military Medical University
2003;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of inducing ossification in necrotic femoral head by VEGF. Methods Healthy rabbits were lesioned into necrotic femoral head by liquid nitrogen and randomly assigned for AdhuVEGF_(121) gene transfection into the necrotic femoral head and AdTrackCMV transfection. The alkaline phosphatase (ALP) activity before transfection and day 1, week 1, 2, 4, 6, 8, 12 after transfection and the expression of collagen Ⅰ at week 2, 4, 8, 12 was examined by enzymology and immunohistochemical techniques. Results The ALP activity of the AdhuVEGF_(121) group was significantly higher than AdTrackCMV group (P
