Regularity of change in gene expression of Toll-like receptor 4 and myeloid differentiation-2 mRNA in the liver of septic rats
- VernacularTitle:脓毒症大鼠肝脏组织Toll样受体4和髓样分化蛋白-2基因表达的变化规律
- Author:
Xiaohui DU
;
Yongming YAO
;
Rong LI
- Publication Type:Journal Article
- Keywords:
Toll-like receptor 4;
myeloid differenti ation protein-2;
sepsis;
tumor necrosis factor
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in Toll-like re ceptor 4 (TLR4) and myeloid differential protein-2 (MD-2) gene expression in t he liver of septic rats, and to elucidate the relationship between the expressio n of TLR4/MD-2 mRNA and TNF? mRNA. Methods Sepsis model was reproduced in rats by intraperitoneal injection of LPS (5mg/kg). 80 Wistar rats were randomly divided into 4 groups: normal control and 1h, 2h, 6h after LPS in jection (20 rats for each group). Rats were sacrificed at different time points, and the expression of TLR4/MD-2 mRNA and TNF-? mRNA in the liver was measure d by RT-PCR. Results The expressions of TLR4/MD-2 mRNA and TN F-? mRNA were up-regulated, peaking 1h after LPS injection, and then declined gradually 2~6h after LPS challenge. The expressions at every time point were hi gher than that in control group (P
