Preliminary analysis of differentially expressed protein profiles in two primary colonic tumor cell lines
- VernacularTitle:两种原发大肠肿瘤细胞株蛋白质表达谱差异初步分析
- Author:
Huasheng TONG
;
Yali ZHANG
;
Bo JIANG
- Publication Type:Journal Article
- Keywords:
two-dimensional electrophoresis;
colorec tal neoplasms;
proteomics
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differences in protein pro fi le between laterally spreading tumor (LST) cell line and SW480 cell line using t wo-dimensional electrophoresis (2-DE). Methods 2-DE was empl oyed to isolate the total proteins of the two cell lines.The gels were stained with silver, and the differential protein expressions were analyzed with Melanin e 3 software. Results The protein spots of the two cell lines w ere 1285?51(LST) and 1184?47(SW480) respectively,containing 96?7 differential protein spots with 50?6 expressed or obviously increased only in LST cell line and 47?5 in SW480 cell line. Conclusion The protein profiles between LST and SW480 cell lins are different and further analysis on the differ ent interested spots is required to acquire the biology-associated proteins spe cific LST.
