Construction of PGEFP-C1/N and its localization in transfected cell
- VernacularTitle:PGEFP-C1/N载体的构建及其在转染细胞中的初步定位
- Author:
Yang QI
;
Jie SUN
;
Cuirong WEN
- Publication Type:Journal Article
- Keywords:
SARS virus;
N protein;
PGEFP-C1;
A549 ce lls;
VeroE6 cells;
subcellular location
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the subcellular location of S AR S-CoV N protein in mammalian cells. Methods The gene fraction of SARS-CoV was cloned into the PGEFP-C1 plasmid to construct expression vecto r PGEFP-C1/N. The subcellular location of N in A549 and VeroE6 cells was observ ed under fluorescence microscope with the aid of transient transfection techniqu e. The expression of the fusion protein (GFP-N) was detected by Western blot. Results The PGEFP-C1/N was constructed. N protein was localiz ed in the cytoplasm of transfected cells and detected by Western blot. C onclusion N protein was localized in the cytoplasm of mammalian cells.
