Influence of RNA interference on expresssion of vascular endothelial growth factor in human ovarian cancer cells and on cell proliferation
- VernacularTitle:RNA干扰对VEGF在人卵巢癌细胞中表达及细胞增殖的影响
- Author:
Qingwang HAN
- Publication Type:Journal Article
- Keywords:
RNA interference;
vascular endothelial growth factor;
ovarian neoplasms;
proliferation
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of vector-based RNA interference(RNAi) on the expression of vascular endothelial growth factor(VEGF) protein in ovarian cancer cells and on proliferation of overian cancer cells. Methods: A vector for transcribing specific small hairpin RNA(shRNA) targeting VEGF gene was constructed,introduced into ovarian cancer HO-8910 cells by Lipofectamine 2000 and the positive clones were screened by G418. The VEGF protein and mRNA expression level of HO-8910 cells were detected by Western blot and semi-quantitative RT-PCR. MTT method and FCM analysis were used to observe the effect of RNAi on proliferation of ovarian cancer cells. Results: The vector of RNA interference was successfully constructed and VEGF expression was descreased significantly in HO-8910-H1-VEGF cells. The growth of ovarian cancer cells transfected with specific small hairpin RNA expression vector was blocked, with G_(1) phase cells increased and S phase cells decreased. Conclusion: The specific small hairpin RNA targeting VEGF mRNA can inhibit the expression of VEGF and the proliferation of ovarian cancer cells, which provides an experimental basis for treating human tumors with anti-angiogenesis method using RNAi. -
