Hypoxia-induced expression of basic fibroblast growth factor in fetal liver stromal cells
- VernacularTitle:低氧诱导胎肝间质细胞表达碱性成纤维生长因子
- Author:
Gexiu LIU
;
Yuan ZHANG
;
Dongmei HE
;
Guangxiao TAN
- Publication Type:Journal Article
- Keywords:
hypoxia;
basic fibroblast growth factor;
stromal cells;
embryo;
liver
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the expression of basic fibroblast growth factor (bFGF) in fetal liver stromal cell(FLSC) during hypoxia so as to provide evidences for hematopoietic stromal cell therapy of brain ischemia. Methods: FLSCs from 14.5-day-old mouse fetuses were cultured and passaged. Subconfluent cells of the fifth passage were cultured in hypoxia condition (95% N_(2) + 5% CO_(2) ). bFGF mRNA and bFGF protein were detected at 2,4,8,16 and 32 h of culture by semi-quantitative RT-PCR and Western blot, respectively. Results: bFGF mRNA and bFGF protein were very low in normally cultured cells, but increased significantly under the condition of hypoxia. Expression of bFGF mRNA obviously increased at 2 h of hypoxia culture and reached peak at 16 h, with the peak-value being 5.52 folds that of the control (-[32.86?9.57-]% vs -[5.94?2.65-]%,P
