Effects of dexamethasone on proliferation and cell cycle of human breast cancer cell line MCF-7
- VernacularTitle:地塞米松对人乳腺癌细胞系MCF-7细胞增殖及细胞周期的影响
- Author:
Mingjuan XU
- Publication Type:Journal Article
- Keywords:
dexamethasone;
breast neoplasms;
cell proliferation;
cell cycle;
p21/WAF1;
breast cancer
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of dexamethasone (Dex), a synthetic glucocorticoid, on the proliferation and cell cycle of human breast carcinoma cell line MCF-7. Methods: MCF-7 cells were treated with different concentrations of Dex (10~(-10)-10~(-6) mol/L). Cell proliferation was evaluated by viable cell count and alkaline phosphatase( AKP) activity was also determined. The cell cycle was examined with flow cytometry and p21/WAF1 expression was detected with Western blot analysis. Results: Dex inhibited the proliferation of MCF-7 cells, with the cell number being 70% of the control group 5 d after treatment with Dex(10~(-7) mol/L). Dex also induced G_0/G_1 arrest of MCF-7 cells phase. AKP activity increased significantly after treatment with Dex in a time- and dose-dependent manner. Dex had no obvious effect on p21/WAF1 expression. (Conclusion:) Glucocortocoids can obviously inhibit the proliferation of MCF-7 cells and induce G_0/G_1 phase arrest.
