Influence of Swan-like memory connector on osteopontin and osteonectin mRNA expression during experimental fracture healing
- VernacularTitle:天鹅记忆接骨器对实验性骨折愈合时骨桥素和骨连接素mRNA表达的影响
- Author:
Ke REN
- Publication Type:Journal Article
- Keywords:
swan-like shape memory connector;
fracture fixation,internal;
fracture healing;
osteopontin;
osteonectin
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect and mechanism of continuous dynamic pressure stress, which is produced by Swan-like shape memory connector(SMC), on osteopontin and osteonectin mRNA expression during experimental fracture healing.Methods: Bilateral humeral diaphyses of 30 adult rabbits were osteotomized in the middle and then 1 humerus was randomly chosen for internal fixation with SMC and the contralateral side was fixed with 4-hole dynamic compression plate(DCP). Six animals were sacrificed at 2, 7, 14, 28 and 56 days after operation and samples from the fracture gaps were obtained. Histological observation and RT-PCR were employed to study the expression of osteopontin and osteonectin mRNA.Results: In terms of histology, the endochondral bone formation and callus remodelling took place earlier in fractures fixed with SMC than with DCP. Osteopontin and osteonectin mRNA expression levels peaked at 14 and 28 days after the fracture, respectively. There was a delay in the increase and decrease of osteopontin and osteonectin mRNA in DCP fixed cases. Conclusion: The persistent dynamic longitudinal pressure stress produced by SMC may promote the healing process of long bones.
