Effect of Soman and hypoxia on TPK activity and cytokines in PC_(12) cell line
- VernacularTitle:缺氧复合梭曼中毒对PC_(12)细胞中IL-4、IL-8和TPK活性的影响
- Author:
Jiqing ZHAO
- Publication Type:Journal Article
- Keywords:
soman;
hypoxia;
PC_(12) cells;
TPK;
IL-4,IL-8
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of interleukin-4,8 and TPK in PC_(12) cells exposed to hypoxia and soman intoxication.Methods PC_(12) cells were randomized into 4 groups: control,hypoxia only,soman only,hypoxia plus soman.The activity of IL-4,IL-8 was measured by ELISA and the activity of tyrosine protein kinase in cells were detected by RIA.Results The activity of IL-4,IL-8 in PC_(12) cell supernatant and TPK in PC_(12) cell cytoplasm,nucleus were increased in 2 h and reached the highest at 12 h,decreased at 24 h in soman group,correspondingly the activity of IL-4,IL-8 in PC_(12) cell supernatant and TPK in PC_(12) cell cytoplasm,nucleus were increased at 2 h and reached the highest at 6 h,decreased at 12 h in soman plus hypoxia group,all higher than that of control.Conclusion TPK,cytokine and its correlated signal pathway lead to an important modulatory role in the brain damage after combined soman and hypoxia injury.
