Protective effects of acutobin on cerebral ischemia-reperfusion injury in rats
- VernacularTitle:蕲蛇酶对大鼠脑缺血再灌注损伤的保护作用
- Author:
Chun LIN
;
Yang HUANG
;
Jun XU
- Publication Type:Journal Article
- Keywords:
acutase;
reperfusion injury;
TXB_2/6-Keto-PGF_(1?);
vascular endothelial cell;
rat
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To further study the protective effects of acutobin on focal cerebral ischemia -reperfusion injury in rats. Methods Reversible middle cerebral artery occlusion (MCAO) models were produced by intraluminal suture technique, and reperfusion was begun 3 hours after occlusion and lasted 24 h. The extent of neurological deficits was evaluated by Longa' method; The cerebrovascular morphology was observed by electron microscope. The infarct area of brain was measured by 2,3,5-triphenyltetrazolium chloride (TTC) staining technique;The 2～4 U?kg~(-1) doses of Acutobin were administrated i.v. at the beginning of ischemia or reperfusion respectively. Results ① After 3-h occlusion and 24 h reperfusion, the neurological syndromes and the infarct area were showed, the change of cerebrovascular morphology were appeared and the ratio of TXB_2/6-Keto-PGF_(1?) in plasma was increased. ② After different doses of acutobin were administrated at different time respectively, the neurological syndromes were alleviated; the infarct areas of brain were diminished; the hurt of cerebrovascular endothelial cell was lessened and the ratios of TXB_2/6-Keto-PGF_(1?) in plasma were reduced. Conclusion Protective effects of acutobin on cerebral ischemia-reperfusion injury in rats may be related to lessening cerebrovascular injury and balancing the ratio of TXB_2/6-Keto-PGF_(1?)
