Effect of diallyl disulfide on G_1 arrest in human colon cancer cell
- VernacularTitle:二烯丙基二硫对人结肠癌HT-29细胞G_1期的阻滞作用
- Author:
Yousheng HUANG
;
Qi SU
;
Xiutian ZHOU
;
Jian SU
;
Qianjin LIAO
;
Na XIE
;
Ling GE
- Publication Type:Journal Article
- Keywords:
diallyl disulfide;
colon cancer;
HT-29 cell;
cell cycle;
Cyclin E
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of DADS in the cell cycle of HT-29 cells. Methods HT-29 cells growth inhibition were measured by MTT assay and cell counting. Phase distribution of cell cycle was analyzed by flow cytometry. Expression of p21~(Waf1),C-myc,Cyclin E was determined by immunohisto- chemistry analysis. Results MTT assay showed that DADS significantly inhibited HT-29 cells and exhibited a time, dose- dependent model. Adding 60 ?mol?L~(-1) and 120 ?mol?L~(-1) DADS for 24 h suppressed HT-29 growth by 23.1%,45.6% respectively. Cell counting showed that average doubling time retarded from 22.58 h in normal cultured HT-29 cells to 31.2 h in 60umol?L~(-1) DADS experimented HT-29 cells(P
