Study on the inhibitory effect of decorin on rat mesangial cell growth and its signal transduction pathway
- VernacularTitle:饰胶蛋白聚糖对大鼠肾系膜细胞生长的抑制作用及其信号转导途径的研究
- Author:
Xiuyan FENG
;
Zhigang ZHANG
;
Zhonghua ZHAO
;
Qi CHEN
;
Muyi GUO
- Publication Type:Journal Article
- Keywords:
Proteoglycans;
Signal transduction;
Mitogen-activated protein kinases;
p21;
Mesangial Cell
- From:
Chinese Journal of Nephrology
2005;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibitory effect of decorin(DCN) on rat mesangial cell (MsC) growth and on the expression of mitogen-activated protein kinases (MAPKs) and p21 protein. Methods Lipofectin-mediated method was used to transfect DCN vector into MsC. After the screen and identification of transfected MsC, DCN-containing supernatant was collected and added into the culture medium of normal MsC, then flow cytometer was used to detect the cell cycle. Western blot analysis was used to explore the changes of MAPK and p21 protein. Immunofluorescence was adapted to detect the expression of p21 on cultured MsC. Results Compared with normal MsC, the number of G2-M cells treated with DCN-containing supernatant decreased to 35%(P
