Determination of valsartan in human plasma by HPLC with fluorimetric detection
- VernacularTitle:HPLC荧光检测法测定人血浆中缬沙坦的浓度
- Author:
Rongwei ZHAO
;
Jia YU
- Publication Type:Journal Article
- Keywords:
valsartan;
HPLC;
fluorescence;
plasma
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a high performance liquid chromatography (HPLC) method to determine the concentration of valsartan in human plasma. METHODS: Using lrbesartan as internal standard, valsartan in plasma samples was determined by HPLC with liquid-liquid extraction, achieved by the column of Agilent ZORBAXSB-C_ 18( 150 mm? 4.6 mm, 5 ?m) at room temperature. The mobile phase consisted of a mixture acetonitrile : water : phosphoric acid : triethylamine was the ratio of 4060 1.0 1.5 (v/v), pumped at a flow rate of 1.0 ml?min~ -1, the wavelengths of fluorimetric excitation and emission were set at 265 and 378 nm respectively. RESULTS: The drug-free plasma did not interfere with the determination of drugs and internal standard. There was good linear relationships (1/C~2 weighted) between peak area ratio of valsartan to internal standard and C (r= 0.9996) within the range of 25-2 500 ng?ml~ -1. The precision of within-day and between-day was good. The lower limit of quantification was 25 ng?ml~ -1. The analytes reconstituted in the mobile phase were also stable at ambient conditions for at least 24 h. Furthermore, valsartan was stable for at least three freeze thaw cycles. CONCLUSION: The HPLC method can be used to determine the concentration of valsartan in human plasma.
